Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)
![Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)](https://www.abcam.com/ps/products/247/ab247348/Images/ab247348-395390-anti-mapk6erk-3-antibody-ep1720y-western-blot-human-knockout-lysate.jpg "Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1720Y] to MAPK6/ERK3 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free
See all MAPK6/ERK3 primary antibodies -
Description
Rabbit monoclonal [EP1720Y] to MAPK6/ERK3 - BSA and Azide free -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HeLa, 2.4G2, HAP1, A431, C2C12, and HeLa (ab150035) whole cell lysates, and rat brain tissue lysates. IHC-P: Human kidney carcinoma and breast carcinoma tissues.
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General notes
ab247348 is the carrier-free version of ab53277. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247348 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1720Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)All lanes : Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MAPK6 knockout HeLa cell lysate
Lane 3 : 2.4G2 (Rat B cell lymphoma B lymphocyte) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab53277).
Lanes 1-3: Merged signal (red and green). Green - ab53277 observed at 90 kDa. Red - loading control ab8245 observed at 36 kDa.
ab53277 Anti-MAPK6/ERK3 antibody [EP1720Y] was shown to specifically react with 3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264910 (knockout cell lysate ab257526) was used. Wild-type and 3 knockout samples were subjected to SDS-PAGE. ab53277 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)](https://www.abcam.com/ps/products/247/ab247348/Images/ab247348-1-anti-mapk6-erk-3-antibody-ep1720y-immunohistochemistry-kidney-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling MAPK6/ERK3 with Purified ab53277 at 1:100 dilution (13.52 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53277) -
![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)](https://www.abcam.com/ps/products/247/ab247348/Images/ab247348-369851-anti-mapk6erk-3-antibody-ep1720y-bsa-and-azide-free-western-blot-hap1-wt-hap1-mapk6-knockout-hap1-ccne2-knockout-a431.jpg)
Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)All lanes : Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MAPK6 knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab53277).
Lanes 1 - 4: Merged signal (red and green). Green - ab53277 observed at 90 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab53277 was shown to react with MAPK6 in HAP1 wild-type cells in Western blot. Loss of signal was observed when MAPK6 knockout sample was used. HAP1 wild-type and MAPK6 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab53277 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)](https://www.abcam.com/ps/products/247/ab247348/Images/ab247348-4-benefits-of-recombinant-antibodies.png)
Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)