Anti-PKC delta (phospho S299) antibody [EPNCI119] (ab133456)
Key features and details
- Rabbit monoclonal [EPNCI119] to PKC delta (phospho S299)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKC delta (phospho S299) antibody [EPNCI119]
See all PKC delta primary antibodies -
Description
Rabbit monoclonal [EPNCI119] to PKC delta (phospho S299) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Serine 299 of Human PKC delta (Q05655).
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Positive control
- MCF-7 cell lysate treated with Caylculin A; Human transitional cell carcinoma tissue.
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institutes Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
The auto-phosphorylation of PKC delta at pS299 targeted by ab133456 was identified in the following paper: FEBS Lett. 2007 Jul 24;581(18):3377-81. Novel phosphorylation site markers of protein kinase C delta activation. Durgan J1, Michael N, Totty N, Parker PJ.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPNCI119 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma tissue labelling PKC delta phospho S299 with ab133456 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-PKC delta (phospho S299) antibody [EPNCI119] (ab133456) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : MCF-7 cell lysate treated with Caylculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa