Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Metabotropic Glutamate Receptor 5 antibody [N75/33] (ab134271)

Key features and details

  • Mouse monoclonal [N75/33] to Metabotropic Glutamate Receptor 5
  • Suitable for: Flow Cyt, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 5 antibody [N75/33]
    See all Metabotropic Glutamate Receptor 5 primary antibodies

  • Description

    Mouse monoclonal [N75/33] to Metabotropic Glutamate Receptor 5

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Fusion protein, corresponding to cytoplasmic, C terminal amino acids 824-1203 of Rat Metabotropic Glutamate Receptor 5 (NP_058708.1).

  • Positive control

    • Rat brain membrane tissue lysate.

  • General notes

    The clone number has been updated from S75-33 to N75/33, both clone numbers name the same antibody clone.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [N75/33] (ab134271)
    Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [N75/33] (ab134271)
    Anti-Metabotropic Glutamate Receptor 5 antibody [N75/33] (ab134271) at 1/1000 dilution + Rat brain membrane tissue lysate

    Secondary
    Goat anti-mouse IgG HRP

    Predicted band size: 132 kDa

  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 5 antibody [N75/33] (ab134271)
    Flow Cytometry - Anti-Metabotropic Glutamate Receptor 5 antibody [N75/33] (ab134271)
    Overlay histogram showing SH-SH5Y cells stained with ab134271 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134271, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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