Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free
    See all Metabotropic Glutamate Receptor 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat caudate putamen and cerebrum tissue; mouse caudate putamen tissue and cerebrum Flow cyt: SH-SY5Y cells.

  • General notes

    ab256589 is the carrier-free version of ab76316. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256589 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Electron Microscopy - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
    Electron Microscopy - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589) Images courtesy of Professor Richard Weinberg, UNC School of Medicine

    This data was developed using ab76316, the same antibody clone in a different buffer formulation.

    Postembedding immunogold labeling of mouse neocortex using Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316). The tissue was embedded in Lowicryl HM20 resin. 60 nm sections were then cut and mounted on nickel mesh grids before undergoing antigen retrieval for 15 minutes in 0.01 M citrate buffer, pH 6 at 60°C.

    The sections were then blocked in 1% BSA/TBSN pH 7.6 and incubated overnight at room temperature with Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1:250. Sections were washed twice in TBSN pH 7.6, treated with 1% normal donkey serum/TBSN pH 8.2 for 30 minutes, before being incubated with donkey anti-rabbit IgG-Au 10-20 nm at 1:20 for two hours at room temperature.

    Sections were then washed in TBSN pH 8.2, followed by water, before undergoing post-staining with 1% uranyl acetate and Sato’s lead. They were then air dried before being transferred to an oven for 30 minutes at 60°C.

    In these images you can see gold immunoparticles on the postsynaptic density of synapses.

    (TBSN = 0.02M TRIS buffered saline (0.3 N, pH 7.6 or 8.2) with 0.005% Tergitol NP-10)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
    Immunohistochemical analysis of Paraffin-embedded mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
     
    Positive staining on mouse cerebrum.
     
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).
  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
    Flow Cytometry - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)

    Flow cytometric analysis of permeabilized SH-SY5Y cells using ab76316 (red) at 1/20 or a rabbit IgG (ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)

    Immunohistochemical analysis of Paraffin-embedded rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on rat cerebrum.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).

  • Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
    Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)

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