Anti-Metabotropic Glutamate Receptor 5 antibody (ab27190)
Key features and details
- Rabbit polyclonal to Metabotropic Glutamate Receptor 5
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 5 antibody
See all Metabotropic Glutamate Receptor 5 primary antibodies -
Description
Rabbit polyclonal to Metabotropic Glutamate Receptor 5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit
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Immunogen
Synthetic peptide within Human Metabotropic Glutamate Receptor 5 aa 1150 to the C-terminus. The exact sequence is proprietary.
Database link: P41594 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody is an affinity purified IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody (ab27190)Ab27190, at a dilution of 1/50, staining mGluR5 in Paraffin embedded formalin fixed human brain tissue section.
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Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 5 antibody (ab27190)ICC/IF image of ab27190 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27190, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
