Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5 - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free
    See all Metabotropic Glutamate Receptor 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human brain tissue, mouse brain lysate.

  • General notes

    ab219374 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374) This image is courtesy of an Abreview submitted by Carl Hobbs.

    ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).

  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
    Flow Cytometry - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)

    Flow cytometric analysis of permeabilized SH-SY5Y cells using ab76316 (red) at 1/20 or a rabbit IgG (ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)

    Human brain tissue with ab76316 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
    Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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