Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (ab274429)
![Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (ab274429)](https://www.abcam.com/ps/products/274/ab274429/Images/ab274429-397802-anti-gpr56-antibody-epr23908115-western-blot-lysate1.jpg "Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (ab274429)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23908-115] to GPR56 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free
See all GPR56 primary antibodies -
Description
Rabbit monoclonal [EPR23908-115] to GPR56 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain, hippocampus and glioma tissue lysates.
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General notes
This antibody ab274429 [EPR23908-115] is a different clone to ab274428 [EPR23908-109].
ab274429 is the carrier-free version of ab274419. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23908-115 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (ab274429)Anti-GPR56 antibody [EPR23908-115] (ab274419) at 1/1000 dilution + Human glioma tissue lysate at 10 µg
Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?This data was developed using ab274419, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 17932623).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 70 seconds.
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![Western blot - Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (ab274429)](https://www.abcam.com/ps/products/274/ab274429/Images/ab274429-397801-anti-gpr56-antibody-epr23908115-western-blot-lysate.jpg)
Western blot - Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (ab274429)All lanes : Anti-GPR56 antibody [EPR23908-115] (ab274419) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Human lung tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?This data was developed using ab274419, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 17932623).
Negative control: Human lung (PMID: 15674329)
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
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![Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (ab274429)](https://www.abcam.com/ps/products/274/ab274429/Images/ab274429-3-benefits-of-recombinant-antibodies.png)
Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (ab274429)