Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6202] to Cytochrome P450 3A4/CYP3A4 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free
    See all Cytochrome P450 3A4/CYP3A4 primary antibodies

  • Description

    Rabbit monoclonal [EPR6202] to Cytochrome P450 3A4/CYP3A4 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Cross-reactivity with other CYP3A family: This antibody has high posibility of reacting with CYP3A7 based on our internal test.

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver tissue IP: Human liver cell lysates

  • General notes

    ab245774 is the carrier-free version of ab124921 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab245774 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Cytochrome P450 3A4 Mouse and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Cytochrome P450 3A4/CYP3A4 with purified ab124921 at 1/400 dilution (0.35 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124921).

  • Immunoprecipitation - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)
    Immunoprecipitation - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)

    ab124921 (purified) at 1/20 dilution (1ug) immunoprecipitating Cytochrome P450 3A4/CYP3A4 in Human liver lysates.
    Lane 1: Human liver lysates 10ug
    Lane 2 (+): ab124921 & Human liver lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124921 in Human liver lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124921).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)

    ab124921 (unpurified), at a 1/100 dilution, staining Cytochrome P450 3A4/CYP3A4 in paraffin embedded Human liver tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, glycerol and sodium azide (ab124921).

  • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)
    Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)

    HepG2 cells were incubated at 37°C for 48h with vehicle control (0 μM) and different concentrations of nicardipine hydrochloride (ab120531) in DMSO. Increased expression of Cytochrome P450 3A4/CYP3A4 (ab124921) correlates with an increase in nicardipine hydrochloride concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab124921 at 1/10000 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, glycerol and sodium azide (ab124921).

  • OI-RD Scanning - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)
    OI-RD Scanning - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, glycerol and sodium azide (ab124921).

  • Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)
    Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] - BSA and Azide free (ab245774)

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