Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (ab250859)
![Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (ab250859)](https://www.abcam.com/ps/products/250/ab250859/Images/ab250859-402704-anti-scg10-antibody-epr15286-39-bsa-and-azide-free-western-blot.jpg "Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (ab250859)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15286-39] to SCG10 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free
See all SCG10 primary antibodies -
Description
Rabbit monoclonal [EPR15286-39] to SCG10 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250859 is the carrier-free version of ab185956. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250859 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15286-39 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (ab250859)All lanes : Anti-SCG10 antibody [EPR15286-39] (ab185956) at 1/20000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : SH-SY5Y cell lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDaThis data was developed using ab185956, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (ab250859)](https://www.abcam.com/ps/products/250/ab250859/Images/ab250859-1-anti-scg10-antibody-epr15286-39-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (ab250859)This data was developed using ab185956, the same antibody clone in a different buffer formulation.Immunoprecipitation of Human fetal brain lysates using ab185956 (lane 1). Detection of SCG10 utilised ab185956 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. The control utilised PBS instead of Human fetal brain lysates (lane 2). Based on the sequence analysis, ab185956 recognizes two isoforms with the predicted MWs of 21KDa and 22KDa, respectively. -
![Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (ab250859)](https://www.abcam.com/ps/products/250/ab250859/Images/ab250859-3-benefits-of-recombinant-antibodies.png)
Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (ab250859)