Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALK-1 antibody [EPR22985-108] - BSA and Azide free (ab269590)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22985-108] to ALK-1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-ALK-1 antibody [EPR22985-108] - BSA and Azide free
    See all ALK-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22985-108] to ALK-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant Mouse ALK-1 Fc Chimera protein; Mouse lung and placenta lysates; rat lung lysates; bEnd.3 whole cell lysate.

  • General notes

    ab269590 is the carrier-free version of ab263902. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ALK-1 antibody [EPR22985-108] - BSA and Azide free (ab269590)
    Western blot - Anti-ALK-1 antibody [EPR22985-108] - BSA and Azide free (ab269590)
    All lanes : Anti-ALK-1 antibody [EPR22985-108] (ab263902) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Mouse placenta lysate
    Lane 3 : Rat lung lysate
    Lane 4 : Rat placenta lysate
    Lane 5 : bEnd.3 (mouse brain endothelioma) whole cell lysate
    Lane 6 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56,63 kDa
    why is the actual band size different from the predicted?



    Blockinmg and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    There are two isoforms. Different isoforms show different content in different tissues.
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11927558, 7488127).

    Negative control: MEF (PMID: 11927558).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263902).

  • Anti-ALK-1 antibody [EPR22985-108] - BSA and Azide free (ab269590)
    Anti-ALK-1 antibody [EPR22985-108] - BSA and Azide free (ab269590)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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