Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

50 µg 284 784 ₸

Order now and get it on Friday March 05, 2021

Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM260] to Histone H2B (acetyl K23)
Suitable for: ICC/IF, WB
Reacts with: Human, Recombinant fragment

Product name

Anti-Histone H2B (acetyl K23) antibody [RM260]
See all Histone H2B primary antibodies
Description

Rabbit monoclonal [RM260] to Histone H2B (acetyl K23)
Host species

Rabbit
Specificity

ab222770 reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Histone H2B (acetyl K23).
Positive control
- WB: HeLa cell lysate. ICC/IF: HeLa cells.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

ab222770 was purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM260
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K23) (red) using ab222770 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).
Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)

All lanes : Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770) at 0.5 µg/ml

Lane 1 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate
Lane 2 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated
Lane 3 : Recombinant Histone H2B

Predicted band size: 14 kDa
Biological activity - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)

ab222770 specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-mordified Lysine 23 in Histone H2B.
Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com