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Epigenetics and Nuclear Signaling Histones H3 Acetylated

Anti-Histone H3 (mono methyl R2) antibody (ab272136)

Anti-Histone H3 (mono methyl R2) antibody (ab272136)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Histone H3 (mono methyl R2)
  • Suitable for: Dot blot, WB
  • Reacts with: Human, Caenorhabditis elegans
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H3 (mono methyl R2) antibody
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (mono methyl R2)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 (mono methyl R2).
    Database link: Q71DI3

  • Positive control

    • WB: HeLa histone prep lysate, C.elegans embryo lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Acetylated

Images

  • Western blot - Anti-Histone H3 (mono methyl R2) antibody (ab272136)
    Western blot - Anti-Histone H3 (mono methyl R2) antibody (ab272136)
    Anti-Histone H3 (mono methyl R2) antibody (ab272136) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) histone prep lysate at 30 µg

    Secondary
    IRDye800™ rabbit secondary antibody at 1/10000 dilution

    Predicted band size: 15 kDa



    Block: 5% BLOTTO.

  • Western blot - Anti-Histone H3 antibody (ab272136)
    Western blot - Anti-Histone H3 antibody (ab272136)
    Anti-Histone H3 (mono methyl R2) antibody (ab272136) at 1/500 dilution + C.elegans embryo lysate at 30 µg

    Secondary
    IRDye800™ rabbit secondary antibody at 1/10000 dilution

    Predicted band size: 15 kDa



    Block: 5% BLOTTO

  • Dot Blot - Anti-Histone H3 antibody (ab272136)
    Dot Blot - Anti-Histone H3 antibody (ab272136)

    Dot Blot analysis of various methylated Histone H3 forms using ab272136 at 1/1000, followed by Dylight™488 rabbit secondary antibody at 1/10000. 

    Lane 1: R2 NM.

    Lane 2: R2 Me1.

    Lane 3: R2 Me2s.

    Lane 4: R2 Me2a.

    Load: 100, 10, 1 picomoles of peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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