Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20971] to Prealbumin - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, IHC-Fr
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Prealbumin antibody [EPR20971] - BSA and Azide free
    See all Prealbumin primary antibodies

  • Description

    Rabbit monoclonal [EPR20971] to Prealbumin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-Fr: Mouse brain (choroid plexus) tissue.

  • General notes

    Ab230828 is the carrier-free version of ab215202. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230828 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)
    Immunoprecipitation - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)

    Prealbumin was immunoprecipitated from 0.35 mg of mouse plasma with ab215202 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215202 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Mouse plasma 10 µg (Input). 

    Lane 2: ab215202 IP in mouse plasma. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215202 in mouse plasma.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    The band shown at 30 kDa is likely to be a dimer of Prealbumin based on the literature (PMID: 19279259).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215202).

  • Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)
    Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Prealbumin with ab215202 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the hepatic cells of mouse liver tissue section (PMID: 25519307).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215202).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in mouse liver (PMID: 25519307; PMID: 19032346). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215202).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in choroid plexuses (arrows) of mouse cerebrum (PMID: 25758461; PMID: 19032346). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215202).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)
    Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (choroid plexus) tissue labeling Prealbumin with ab215202 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the endothelial cells of choroid plexus, negative staining in the endothelial cells of blood vessels on mouse brain tissue section (PMID: 19539719; 20462362).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215202).

  • Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)
    Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (ab230828)

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