Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17883] to Keratin 12/K12
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Keratin 12/K12 antibody [EPR17883]
See all Keratin 12/K12 primary antibodies
Description

Rabbit monoclonal [EPR17883] to Keratin 12/K12
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human Keratin 12/K12 transfected HEK-293 whole cell lysate; Rat and Mouse eyeball lysates. IHC-P: Human, mouse and rat cornea tissues.
General notes
Previously labelled as Keratin 12.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17883
Isotype

IgG
Research areas

Western blot - Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

All lanes : Anti-Keratin 12/K12 antibody [EPR17883] (ab185626) at 1/20000 dilution

Lane 1 : Empty vector (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Human Keratin 12/K12 transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 2 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

All lanes : Anti-Keratin 12/K12 antibody [EPR17883] (ab185626) at 1/2000 dilution

Lane 1 : Rat eyeball lysate
Lane 2 : Mouse eyeball lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : Mouse heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : Mouse spleen lysate
Lane 8 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 9 : Y79 (Human retinoblastoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 54 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Keratin 12/K12 is cornea specific. [PMID: 8759347].
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

Immunohistochemical analysis of paraffin-embedded Human cornea tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on epithelium of Human cornea is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on Human kidney.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

Immunohistochemical analysis of paraffin-embedded sagittal section of mouse cornea tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on epithelium of mouse cornea is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on mouse cerebrum.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

Immunohistochemical analysis of paraffin-embedded sagittal section of Rat cornea tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on epithelium of rat cornea is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on rat liver.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

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