Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

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Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17882] to Keratin 12/K12
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Keratin 12/K12 antibody [EPR17882]
See all Keratin 12/K12 primary antibodies
Description

Rabbit monoclonal [EPR17882] to Keratin 12/K12
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Keratin 12/K12 transfected HEK-293 whole cell lysate; Mouse and rat eyeball lysates. IHC-P: Mouse and rat cornea tissues. ICC/IF: Keratin 12/K12 transfected HeLa cells.
General notes
Previously labelled as Keratin 12.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EPR17882
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)

Immunohistochemical analysis of paraffin-embedded Mouse cornea tissue labeling Keratin 12/K12 with ab185627 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on the mouse corneal epithelium is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)

Immunohistochemical analysis of paraffin-embedded Rat cornea tissue labeling Keratin 12/K12 with ab185627 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on the rat corneal epithelium is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Keratin 12/K12 with ab185627 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing no staining on HeLa cell line.
The expression increased after transfection of Keratin 12/K12. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows:-

-ve control 1: ab185627 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Western blot - Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)

All lanes : Anti-Keratin 12/K12 antibody [EPR17882] (ab185627) at 1/100000 dilution

Lane 1 : Empty vector (vector control) transfected HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : Keratin 12/K12 transfected HEK-293 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)

All lanes : Anti-Keratin 12/K12 antibody [EPR17882] (ab185627) at 1/5000 dilution

Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : Y79 (Human retinoblastoma cell line) whole cell lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : Rat brain lysate
Lane 8 : Rat heart lysate
Lane 9 : Rat kidney lysate
Lane 10 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Keratin 12/K12 is expressed specifically in corneal epithelium (Am J Hum Genet. 1997. 61, 1268–1275).
Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)

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