Anti-Cytokeratin 14 antibody [EPR17336] - BSA and Azide free (ab251249)
![Anti-Cytokeratin 14 antibody [EPR17336] - BSA and Azide free (ab251249)](https://www.abcam.com/ps/products/251/ab251249/Images/ab251249-369863-anti-cytokeratin-14-antibody-epr17336-bsa-and-azide-free-western-blot-a431-wt-a431-krt14-knockout-human-skin-mcf7.jpg "Anti-Cytokeratin 14 antibody [EPR17336] - BSA and Azide free (ab251249)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17336] to Cytokeratin 14 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cytokeratin 14 antibody [EPR17336] - BSA and Azide free
See all Cytokeratin 14 primary antibodies -
Description
Rabbit monoclonal [EPR17336] to Cytokeratin 14 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 cell lysate, mouse and rat skin lysates and human skin tissue lysates. IHC-P: Human squamous cell carcinoma of cervix and mouse skin tissues. ICC/IF: HaCaT and A431 cells.
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General notes
ab251249 is the carrier-free version of ab197893. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17336 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytokeratin 14 antibody [EPR17336] - BSA and Azide free (ab251249)All lanes : Anti-Cytokeratin 14 antibody [EPR17336] (ab197893) at 1/50000 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : KRT14 knockout A431 whole cell lysate
Lane 3 : Human skin whole tissue lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab197893).
Lanes 1 - 4: Merged signal (red and green). Green - ab197893 observed at 52 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab197893 was shown to react with KRT14 in A431 wild-type cells in Western blot. Loss of signal was observed when KRT14 knockout sample was used. A431 wild-type and KRT14 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab197893 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 50000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Cytokeratin 14 antibody [EPR17336] - BSA and Azide free (ab251249)](https://www.abcam.com/ps/products/251/ab251249/Images/ab251249-2-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 14 antibody [EPR17336] - BSA and Azide free (ab251249)