Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121)
Key features and details
- Rabbit polyclonal to Cytokeratin 5 - Cytoskeleton Marker
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 5 antibody - Cytoskeleton Marker
See all Cytokeratin 5 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 5 - Cytoskeleton Marker -
Host species
Rabbit -
Specificity
ab53121 detects endogenous levels of total Cytokeratin 5 protein. -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Cytokeratin 5.
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Positive control
- HepG2 cell extracts and human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.00
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53121 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121)All lanes : Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121) at 1/300 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size: 62 kDa
Observed band size: 62 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121)ab53121 at 1/50 dilution staining Cytokeratin 5 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121)ICC/IF image of ab53121 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53121, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
