Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 16/K16 antibody [LL025] - BSA and Azide free (ab256136)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [LL025] to Cytokeratin 16/K16 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 16/K16 antibody [LL025] - BSA and Azide free
    See all Cytokeratin 16/K16 primary antibodies

  • Description

    Mouse monoclonal [LL025] to Cytokeratin 16/K16 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cytokeratin 16/K16. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P08779

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human esophagus and tonsil tissue.

  • General notes

    ab256136 is the carrier-free version of ab8741. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256136 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [LL025] - BSA and Azide free (ab256136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [LL025] - BSA and Azide free (ab256136)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab8741).

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16/K16 with ab8741 at 2.74µg/ml, followed by ready to use secondary. Positive staining on human esophagus tissue is observed. The section was incubated with ab8741 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [LL025] - BSA and Azide free (ab256136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [LL025] - BSA and Azide free (ab256136)

    This image was produced using the same antibody clone but in a different formulation containig PBS, sodium azide, glycerol and BSA (ab8741).

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 16/K16 with ab8741 at 2.74µg/ml, followed by ready to use secondary. Positive staining on epithelial cells of human tonsil tissue is observed. The section was incubated with ab8741 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Anti-Cytokeratin 16/K16 antibody [LL025] - BSA and Azide free (ab256136)
    Anti-Cytokeratin 16/K16 antibody [LL025] - BSA and Azide free (ab256136)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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