Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511)
Key features and details
- Rabbit polyclonal to Cytokeratin 20 - Cytoskeleton Marker
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 20 antibody - Cytoskeleton Marker
See all Cytokeratin 20 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 20 - Cytoskeleton Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 75-287 of Human Cytokeratin 20 (NP_061883).
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Positive control
- HeLa cell, HT29 xenograft
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Ab97511 staining Cytokeratin 20 in Mouse urinary bladder by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Sample was incubated with primary antibody at a 1:500 dilution.
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Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
Ab97511 staining Cytokeratin 20 in HT29 xenograft by Immunohistochemistry (IHC-P - paraformaldehyde - fixed, paraffin embedded sections). Samples were incubated with primary antibody at 1:100 dilution.
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Immunofluorescence analysis of Cytokeratin 20 in paraformaldehyde fixed HeLa, using ab97511 at a 1/200 dilution.
Lower image (2): merged with DNA probe.