Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)
![Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)](https://www.abcam.com/ps/products/243/ab243857/Images/ab243857-2-243440IHC2.jpg "Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22339-65] to BNP - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-BNP antibody [EPR22339-65] - BSA and Azide free
See all BNP primary antibodies -
Description
Rabbit monoclonal [EPR22339-65] to BNP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: IHC-Fr or WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse and rat heart tissues.
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General notes
Ab243857 is the carrier-free version of ab243440. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab243857 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22339-65 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)
Immunohistochemical analysis of paraffin-embedded rat heart tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the atrium (PMID: 15576492). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243440).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)](https://www.abcam.com/ps/products/243/ab243857/Images/ab243857-1-243440IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the atrium (PMID: 15576492). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243440).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)](https://www.abcam.com/ps/products/243/ab243857/Images/ab243857-4-243440IHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.
Negative tissue: no staining on rat liver (PMID: 15576492).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243440).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)](https://www.abcam.com/ps/products/243/ab243857/Images/ab243857-3-243440IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.
Negative tissue: no staining on mouse liver (PMID: 15576492).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243440).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)](https://www.abcam.com/ps/products/243/ab243857/Images/ab243857-5-benefits-of-recombinant-antibodies.png)
Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)
