Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)
![Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)](https://www.abcam.com/ps/products/278/ab278723/Images/ab278723-2-mek12s217s221h2daudi.jpg "Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MEK12S217S221-H2] to MEK1 + MEK2 (phospho S217 + S221)
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2]
See all MEK1 + MEK2 primary antibodies -
Description
Rabbit monoclonal [MEK12S217S221-H2] to MEK1 + MEK2 (phospho S217 + S221) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human MEK1 + MEK2 (phospho S217 + S221). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Also: Uniprot P36507
Database link: Q02750 -
Positive control
- WB: HeLa (treated with UV + TPA) cell extract. Flow cyt: Daudi cells (treated with IFNa + IL-4 + pervanadate).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
MEK12S217S221-H2 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using ab278723 at 0.01 µg/mL.
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![Western blot - Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)](https://www.abcam.com/ps/products/278/ab278723/Images/ab278723-5-ab278723comparisonwb.jpg)
Western blot - Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)All lanes : Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723) at 0.1 µg/ml
Lane 1 : HeLa cell extract treated with UV+TPA
Lane 2 : HeLa cell extract untreated with UV+TPA
Predicted band size: 43 kDa
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![Flow Cytometry - Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)](https://www.abcam.com/ps/products/278/ab278723/Images/ab278723-4-mek12s217s221h2pepblock.jpg)
Flow Cytometry - Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or stained with isotype control (orange) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) or untreated and blocked with phospho peptide (gold) or treated and blocked with phospho peptide (black) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278723 at 0.01 µg/mL.
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![Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)](https://www.abcam.com/ps/products/278/ab278723/Images/ab278723-1-benefits-of-recombinant-antibodies.png)
Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)