Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [MEK12S217S221-H2] to MEK1 + MEK2 (phospho S217 + S221)
Suitable for: WB, Flow Cyt
Reacts with: Human

Product name

Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2]
See all MEK1 + MEK2 primary antibodies
Description

Rabbit monoclonal [MEK12S217S221-H2] to MEK1 + MEK2 (phospho S217 + S221)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MEK1 + MEK2 (phospho S217 + S221). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Also: Uniprot P36507
Database link: Q02750

Run BLAST with Run BLAST with
Positive control
- WB: HeLa (treated with UV + TPA) cell extract. Flow cyt: Daudi cells (treated with IFNa + IL-4 + pervanadate).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

MEK12S217S221-H2
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using ab278723 at 0.01 µg/mL.
Western blot - Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)

All lanes : Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723) at 0.1 µg/ml

Lane 1 : HeLa cell extract treated with UV+TPA
Lane 2 : HeLa cell extract untreated with UV+TPA

Predicted band size: 43 kDa
Flow Cytometry - Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)

Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or stained with isotype control (orange) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) or untreated and blocked with phospho peptide (gold) or treated and blocked with phospho peptide (black) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278723 at 0.01 µg/mL.
Anti-MEK1 + MEK2 (phospho S217 + S221) antibody [MEK12S217S221-H2] (ab278723)

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