Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)
![Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)](https://www.abcam.com/ps/products/52/ab52855/Images/ab52855-65297-anti-cbl-phospho-y731-antibody-ep973y-western-blot.gif "Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)")
Key features and details
- Rabbit monoclonal [EP973Y] to CBL (phospho Y731)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CBL (phospho Y731) antibody [EP973Y]
See all CBL primary antibodies -
Description
Rabbit monoclonal [EP973Y] to CBL (phospho Y731) -
Host species
Rabbit -
Specificity
ab52855 detects CBL phosphorylated on Tyrosine 731. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyr731 of human CBL.
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Positive control
- Raji cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP973Y -
Isotype
IgG -
Research areas
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
Images
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Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)All lanes : Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855) at 1/2000 dilution
Lane 1 : Raji cell lysate untreated
Lane 2 : Raji cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
