Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
![Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)](https://www.abcam.com/ps/products/2/ab2182/Images/ab2182-5450-anti-bestrophin-antibody-e6-6-western-blot.jpg "Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)")
Key features and details
- Mouse monoclonal [E6-6] to Bestrophin/BEST1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Bestrophin/BEST1 antibody [E6-6]
See all Bestrophin/BEST1 primary antibodies -
Description
Mouse monoclonal [E6-6] to Bestrophin/BEST1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Bestrophin/BEST1 aa 568-585 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:KDHMDPYWALENRDEAHS
Database link: O76090 -
Positive control
- IHC-Fr: Pig retinal pigment epithelium tissue. ICC/IF: Bovine retinal pigment epithelium (RPE). WB: Human RPE cell lysate.
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General notes
This product was previously labelled as Bestrophin
This product has switched from ascites to TCS on 09th September 2020.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: Ascites -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
E6-6 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution + Human RPE cell lysate
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![Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)](https://www.abcam.com/ps/products/2/ab2182/Images/ab2182-5452-anti-bestrophin-antibody-e6-6-western-blot.jpg)
Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) This image is courtesy of an Abreview submitted by Dr Vladimir MilenkovicAll lanes : Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution
Lane 1 : Human RPE, retinal pigment epithelial cell lysate
Lane 2 : Non transfected HEK 293 cell extract
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-mouse
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 67 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThe primary antobody was diluted in PBS/Tween/5%Milk and incubated for 1.5 hours at 25°C.
