Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)

Key features and details

  • Mouse monoclonal [E6-6] to Bestrophin/BEST1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Bestrophin/BEST1 antibody [E6-6]
    See all Bestrophin/BEST1 primary antibodies

  • Description

    Mouse monoclonal [E6-6] to Bestrophin/BEST1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Bestrophin/BEST1 aa 568-585 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    KDHMDPYWALENRDEAHS


    Database link: O76090
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-Fr: Pig retinal pigment epithelium tissue. ICC/IF: Bovine retinal pigment epithelium (RPE). WB: Human RPE cell lysate.

  • General notes

     This product was previously labelled as Bestrophin

     This product has switched from ascites to TCS on 09th September 2020.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: Ascites
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    E6-6

  • Myeloma

    unknown

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
    Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
    Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution + Human RPE cell lysate
  • Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
    Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
    All lanes : Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution

    Lane 1 : Human RPE, retinal pigment epithelial cell lysate
    Lane 2 : Non transfected HEK 293 cell extract

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-mouse

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 67 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    The primary antobody was diluted in PBS/Tween/5%Milk and incubated for 1.5 hours at 25°C.

    See Abreview

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