Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20822] to BHMT + BHMT2
Suitable for: WB, IHC-P
Reacts with: Rat, Human

Product name

Anti-BHMT + BHMT2 antibody [EPR20822]
Description

Rabbit monoclonal [EPR20822] to BHMT + BHMT2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Rat Human
WB	Rat Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged human BHMT2 and BHMT recombinant proteins; Human fetal liver and fetal kidney tissue lysate; Rat liver lysate. IHC-P: Human kidney and liver tissues; Rat liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20822
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling BHMT + BHMT2 with ab213491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human kidney tissue is observed (PMID: 9281325; PMID: 26592251). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC note: 1:20,000, recommend human and rat. BHMT2 involved in the regulation of homocysteine metabolism. It’s mainly expressed on liver and kidney and its subcellular location was cytoplasm. IHC results showed specific staining on liver and kidney of human and liver, mouse kidney was very weak staining and testis leydig cells were non-specific staining. So IHC recommend human and rat.

Note: It can show nuclear staining if working at higher concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BHMT + BHMT2 with ab213491 at a 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human liver is observed (PMID: 9281325; PMID: 26592251). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BHMT + BHMT2 with ab213491 at 1/20,000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat liver is observed (PMID: 9281325; PMID: 26592251).counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

All lanes : Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution

Lane 1 : Recombinant Human BHMT2 protein (denatured) (ab180277)
Lane 2 : Recombinant Human BHMT protein (ab51280)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 40 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Lane 1 and 2 samples loaded at 10 ng.

Exposure time: 58 seconds.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

All lanes : Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution

Lane 1 : Human fetal liver tissue
Lane 2 : Human fetal kidney tissue
Lane 3 : Human fetal spleen tissue

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 40 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 3 minutes

BHMT and BHMT2 are mainly expressed in liver and kidney, negative in spleen and other tissues (PMID18457970; PMID: 11087663). The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution + Rat liver tissue at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 40 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 7 seconds.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

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