Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20822] to BHMT + BHMT2
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-BHMT + BHMT2 antibody [EPR20822]

  • Description

    Rabbit monoclonal [EPR20822] to BHMT + BHMT2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    WB
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human BHMT2 and BHMT recombinant proteins; Human fetal liver and fetal kidney tissue lysate; Rat liver lysate. IHC-P: Human kidney and liver tissues; Rat liver tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling BHMT + BHMT2 with ab213491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human kidney tissue is observed (PMID: 9281325; PMID: 26592251). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    IHC note: 1:20,000, recommend human and rat. BHMT2 involved in the regulation of homocysteine metabolism. It’s mainly expressed on liver and kidney and its subcellular location was cytoplasm. IHC results showed specific staining on liver and kidney of human and liver, mouse kidney was very weak staining and testis leydig cells were non-specific staining. So IHC recommend human and rat.

    Note: It can show nuclear staining if working at higher concentration.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BHMT + BHMT2 with ab213491 at a 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human liver is observed (PMID: 9281325; PMID: 26592251). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BHMT + BHMT2 with ab213491 at 1/20,000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat liver is observed (PMID: 9281325; PMID: 26592251).counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    All lanes : Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution

    Lane 1 : Recombinant Human BHMT2 protein (denatured) (ab180277)
    Lane 2 : Recombinant Human BHMT protein (ab51280)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Lane 1 and 2 samples loaded at 10 ng.

    Exposure time: 58 seconds.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
  • Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    All lanes : Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue
    Lane 2 : Human fetal kidney tissue
    Lane 3 : Human fetal spleen tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 3 minutes

    BHMT and BHMT2 are mainly expressed in liver and kidney, negative in spleen and other tissues (PMID18457970; PMID: 11087663). The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution + Rat liver tissue at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 7 seconds.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)
    Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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