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Human Beta-tubulin ELISA Kit (ab245722)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 25.77 pg/ml
  • Range: 187.5 pg/ml - 12000 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Beta-tubulin ELISA Kit
    See all beta Tubulin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    cell extract 5 3.4%
    Inter-assay
    Sample n Mean SD CV%
    cell extract 3 4%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    25.77 pg/ml

  • Range

    187.5 pg/ml - 12000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 98 92% - 103%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Beta-tubulin ELISA Kit (ab245722) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta-tubulin protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Beta-tubulin with 25.77 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Beta-tubulin Capture Antibody 1 x 600µl
    10X Human Beta-tubulin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Human Beta-tubulin Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

  • Tissue specificity

    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

  • Involvement in disease

    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1

  • Sequence similarities

    Belongs to the tubulin family.

  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.

  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Information by UniProt

  • Alternative names

    • Beta 4 tubulin
    • Beta 5 tubulin
    • beta Ib tubulin
    • Beta1 tubulin
    • Class I beta tubulin
    • M40
    • MGC117247
    • MGC16435
    • OK/SW cl.56
    • OK/SWcl.56
    • TBB5_HUMAN
    • TUBB
    • TUBB 1
    • TUBB 2
    • TUBB 5
    • TUBB1
    • TUBB2
    • TUBB5
    • tubulin beta 1 chain
    • Tubulin beta 2 chain
    • tubulin beta 5 chain
    • Tubulin beta chain
    • Tubulin beta class I
    • tubulin beta polypeptide
    • Tubulin beta-5 chain
    see all

  • Database links

    Images

    • Other - Human Beta-tubulin ELISA Kit (ab245722)
      Other - Human Beta-tubulin ELISA Kit (ab245722)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human Beta-Tubulin standard curve in 1X Cell Extraction Buffer PTR.
      Example of human Beta-Tubulin standard curve in 1X Cell Extraction Buffer PTR.
      The Beta-Tubulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native Beta-Tubulin in human HeLa and 293 cell extract samples based on a 2 µg/mL extract load and Jurkat cell extract samples based on a 1 µg/mL extract load.
      Interpolated concentrations of native Beta-Tubulin in human HeLa and 293 cell extract samples based on a 2 µg/mL extract load and Jurkat cell extract samples based on a 1 µg/mL extract load.
      The concentrations of Beta-Tubulin were measured in duplicate and interpolated from the Beta-Tubulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-Tubulin concentration was determined to be 8,281.0 pg/mL in HeLa, 3,557.9 pg/ml in Jurkat, and 6,718.6 pg/ml in 293 cell extract samples at the specified extract loads.
    • Sandwich ELISA - Human Beta-tubulin ELISA Kit (ab245722)
      Sandwich ELISA - Human Beta-tubulin ELISA Kit (ab245722)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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