Anti-Doublecortin antibody (ab235153)
Key features and details
- Goat polyclonal to Doublecortin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Doublecortin antibody
See all Doublecortin primary antibodies -
Description
Goat polyclonal to Doublecortin -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells. WB: Post-natal rat pup whole brain lysate (PND2-6)* and rat pup PND2-6 – minimal cortex brain lysate. Doublecortin (human) overexpressing HEK-293T lysate C-Myc/DDK tagged. IHC-P: Mouse dentate gyrus tissue. Human brain tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody (ab235153)
4% PFA fixed, 0.3% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells labeling Doublecortin using ab235153 at 15 µg/ml (O/N at 4°C) (green) in ICC/IF.The Secondary antibody is a Donkey anti-Gt IgG DyLigh™ 488 conjugated preadsorbed antibody, used at 5 µg/ml for 1 hour at room temperature. The Nuclear counterstain is DAPI (Blue).
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Western blot - Anti-Doublecortin antibody (ab235153)All lanes : Anti-Doublecortin antibody (ab235153) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg
Lane 2 : Post-natal rat pup whole brain lysate (PND2-6)* at 15 µg
Lane 3 : Rat pup PND2-6 - minimal cortex brain lysate at 15 µg
Lane 4 : Rat pup PND2-6 – minimal cortex brain lysate at 35 µg
Secondary
Lanes 1-2 & 4 : Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution
Predicted band size: 49 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds* "Doublecortin microtubule affinity is regulated by a balance of kinase and phosphatase activity at the leading edge of migrating neurons." Schaar B.T., Kinoshita K., McConnell S.K. Neuron 41:203213 (2004).
Minimal cortex defined: small portion of cortex removed prior to lysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab235153)Formalin-fixed, paraffin-embedded mouse dentate gyrus tissue stained for Doublecortin with ab235153 at a 1/200 dilution (30 mins at room temperature) in immunohistochemical analysis. A Donkey anti Goat (HRP) secondary was used at 4 µl/ml (20 mins at room temperature). Counterstained with hematoxylin. HIER using citrate buffer for 20 mins.
Pathologist analysis: Doublecortin (DCX) showed selective staining of soma and nuclei of cells in the dentate gyrus in mouse brain. The specificity of the staining is consistent with DCX staining in the Human Protein Atlas and as such, is suitable for IHC at a concentration of 1/200.
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Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody (ab235153)4% PFA fixed, 0.3% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells labeling Doublecortin using ab235153 at 15 µg/ml (O/N at 4°C) (green) in ICC/IF. The Secondary antibody is a Donkey anti-Gt IgG DyLigh™ 488 conjugated preadsorbed, used at 5 µg/ml for 1 hour at room temperature. The Nuclear counterstain is DAPI (Blue).
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Western blot - Anti-Doublecortin antibody (ab235153)All lanes : Anti-Doublecortin antibody (ab235153) at 1/1000 dilution
Lane 1 : Doublecortin (human) overexpressing HEK-293T lysate C-Myc/DDK tagged
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Adult mouse normal brain tissue lysate
Lane 4 : Adult rat normal brain tissue lysate
Secondary
All lanes : Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution
Predicted band size: 49 kDa
Observed band size: 48, 35 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab235153)Human brain tissue stained for Doublecortin using ab235153 at 1/1000 dilution in immunohistochemical analysis. The counter stain is hematoxylin.
Heat induced epitope retrieval (HIER).