Anti-MRas antibody [EPR12457] (ab176570)
![Anti-MRas antibody [EPR12457] (ab176570)](https://www.abcam.com/ps/products/176/ab176570/Images/ab176570-395376-anti-mras-antibody-epr12457-western-blot-human-knockout-lysate.jpg "Anti-MRas antibody [EPR12457] (ab176570)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12457] to MRas
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-MRas antibody [EPR12457]
See all MRas primary antibodies -
Description
Rabbit monoclonal [EPR12457] to MRas -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MRas aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O14807 -
Positive control
- WB: HeLa cell lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12457 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MRas antibody [EPR12457] (ab176570)All lanes : Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MRAS knockout HeLa cell lysate
Lane 3 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab176570 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.
ab176570 Anti-MRas antibody [EPR12457] was shown to specifically react with MRas in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265510 (knockout cell lysate ab257541) was used. Wild-type and MRas knockout samples were subjected to SDS-PAGE. ab176570 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Anti-MRas antibody [EPR12457] (ab176570)](https://www.abcam.com/ps/products/176/ab176570/Images/ab176570-4-benefits-of-recombinant-antibodies.png)
Anti-MRas antibody [EPR12457] (ab176570)
