Anti-MRas antibody (ab26303)
Key features and details
- Rabbit polyclonal to MRas
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MRas antibody
See all MRas primary antibodies -
Description
Rabbit polyclonal to MRas -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide:
KKKKTKWRGDRATGTHKLQ
, corresponding to C terminal amino acids 186-204 of Human MRas
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MRas antibody (ab26303)ICC/IF image of ab26303 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26303, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-MRas antibody (ab26303)All lanes : Anti-MRas antibody (ab26303)
Lane 1 : HEK 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate
Secondary
All lanes : anti-rabbit HRP conjugated
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Observed band larger than predicted size, thought to be due to post-translational modifications.
