Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DMGDH antibody [EPR16908] (ab198292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16908] to DMGDH
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-DMGDH antibody [EPR16908]
    See all DMGDH primary antibodies

  • Description

    Rabbit monoclonal [EPR16908] to DMGDH

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, fetal liver and HepG2 cell lysates; Human liver and kidney tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.
    Note: Cytoplasm staining on human kidney tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
    Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
    All lanes : Anti-DMGDH antibody [EPR16908] (ab198292) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa


    Exposure time: 2 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
    Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
    Anti-DMGDH antibody [EPR16908] (ab198292) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.
    Note: Cytoplasm staining on human liver tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (ab198292)
    Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (ab198292)

    DMGDH protein was immunoprecipitation from 1mg of Human fetal liver whole cell lysate with ab198292 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, Human fetal liver whole cell lysate, 10ug. Lane 2: IP of DMGDH from Human fetal liver whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198292 in Human fetal liver whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-DMGDH antibody [EPR16908] (ab198292)
    Anti-DMGDH antibody [EPR16908] (ab198292)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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