Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16590] to Choline Acetyltransferase
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Rabbit, Guinea pig

Product name

Anti-Choline Acetyltransferase antibody [EPR16590]
See all Choline Acetyltransferase primary antibodies
Description

Rabbit monoclonal [EPR16590] to Choline Acetyltransferase
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Guinea pig
WB	Mouse Rat Guinea pig

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat brain lysate; Rabbit brain lysate; Guinea Pig brain and cerebellum lysates. IHC-P: Mouse cerebral cortex tissue, Guinea Pig cerebral cortex tissue,
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16590
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

All lanes : Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/5000 dilution

Lane 1 : Guinea Pig brain lysate
Lane 2 : Guinea Pig cerebellum lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 72 kDa
Observed band size: 72 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) This image is courtesy of an anonymous abreview.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal.
See Abreview
Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution + Rabbit brain lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 72 kDa
Observed band size: 72 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution + Rat brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 72 kDa
Observed band size: 72 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/2000 dilution + Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 72 kDa
Observed band size: 72 kDa

Exposure time: 3 minutes
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

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