Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (ab279710)
![Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (ab279710)](https://www.abcam.com/ps/products/279/ab279710/Images/ab279710-1-anti-vacht-antibody-epr24248167-immunohistochemistry-mouse-cerebrum.jpg "Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (ab279710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24248-167] to VAChT - BSA and Azide free
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Rat
Overview
-
Product name
Anti-VAChT antibody [EPR24248-167] - BSA and Azide free
See all VAChT primary antibodies -
Description
Rabbit monoclonal [EPR24248-167] to VAChT - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details
Unsuitable for: ICC/IF,IHC-Fr or WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Mouse cerebrum; Rat cerebrum. IP: Mouse brain tissue lysate; Rat brain tissue lysate
-
General notes
ab279710 is the carrier-free version of ab271111.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24248-167 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)
This data was developed using ab271111, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Punctate staining in mouse cerebrum. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)This data was developed using ab271111, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly punctate staining in the corpus striatum of mouse cerebrum. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunoprecipitation - Anti-VAChT antibody - BSA and Azide free (ab279710)This data was developed using ab271111, the same antibody clone in a different buffer formulation.
VAChT was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab271111 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271111 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab271111 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271111 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)This data was developed using ab271111, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Punctate staining in rat cerebrum. The section was incubated with ab271111 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunoprecipitation - Anti-VAChT antibody - BSA and Azide free (ab279710)This data was developed using ab271111, the same antibody clone in a different buffer formulation.
VAChT was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab271111 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271111 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate 10 ug
Lane 2: ab271111 IP in Rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271111 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)This data was developed using ab271111, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in mouse liver (PMID: 9427309). The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)This data was developed using ab271111, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in rat liver. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
