Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (ab279710)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24248-167] to VAChT - BSA and Azide free
  • Suitable for: IP, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-VAChT antibody [EPR24248-167] - BSA and Azide free
    See all VAChT primary antibodies

  • Description

    Rabbit monoclonal [EPR24248-167] to VAChT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: ICC/IF,IHC-Fr or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebrum; Rat cerebrum. IP: Mouse brain tissue lysate; Rat brain tissue lysate

  • General notes

    ab279710 is the carrier-free version of ab271111.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)

    This data was developed using ab271111, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Punctate staining in mouse cerebrum. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)

    This data was developed using ab271111, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly punctate staining in the corpus striatum of mouse cerebrum. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-VAChT antibody - BSA and Azide free (ab279710)
    Immunoprecipitation - Anti-VAChT antibody - BSA and Azide free (ab279710)

    This data was developed using ab271111, the same antibody clone in a different buffer formulation.

    VAChT was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab271111 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271111 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab271111 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271111 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)

    This data was developed using ab271111, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Punctate staining in rat cerebrum. The section was incubated with ab271111 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-VAChT antibody - BSA and Azide free (ab279710)
    Immunoprecipitation - Anti-VAChT antibody - BSA and Azide free (ab279710)

    This data was developed using ab271111, the same antibody clone in a different buffer formulation.

    VAChT was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab271111 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271111 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 ug

    Lane 2: ab271111 IP in Rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271111 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)

    This data was developed using ab271111, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in mouse liver (PMID: 9427309). The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody - BSA and Azide free (ab279710)

    This data was developed using ab271111, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in rat liver. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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