Butyrylcholinesterase Assay Kit (ab241010)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
Overview
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Product name
Butyrylcholinesterase Assay Kit
See all Butyrylcholinesterase kits -
Detection method
Colorimetric -
Product overview
Butyrylcholinesterase Assay Kit (ab241010) is based on the ability of Butyrylcholinesterase (BChE) to hydrolyze substrate and produce thiocholine. Thiocholine reacts with 5, 5’-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be quantified at 412 nm. The assay is simple, sensitive and can detect as low as 0.2 U/ml in variety of samples.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests BChE Assay Buffer 1 x 50ml BChE Substrate (in DMSO) 1 x 100µl Butyrylcholinesterase 1 vial DTNB 1 vial TNB Standard 1 vial -
Research areas
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Function
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. -
Tissue specificity
Detected in blood plasma (at protein level). Present in most cells except erythrocytes. -
Involvement in disease
Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acylcholine acylhydrolase
- BCHE
- Butyrylcholine esterase
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Images
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TNB standard curve
TNB standard curve
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BChE activityBChE activity in Human Blood (10 µL, 1:100 dilution), Human Serum (10 µL, 1:50 dilution) and Human Plasma (10 µL, 1:50 dilution).
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BChE activityBChE activity in Rat Liver (30 µg protein) and Rat Lung (15 µg protein). Assays were performed following the kit protocol.