Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23792-163] to Butyrylcholinesterase - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free
    See all Butyrylcholinesterase primary antibodies

  • Description

    Rabbit monoclonal [EPR23792-163] to Butyrylcholinesterase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse liver, brain, heart and lung tissue lysates; Rat liver and brain tissue lysates.

  • General notes

    ab274382 is the carrier-free version of ab274375. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)
    Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)
    All lanes : Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse lung tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab274375, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure times: Lane 1-3: 89 seconds Lane 4: 3 minutes.

  • Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)
    Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)
    All lanes : Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution

    Lane 1 : Rat liver tissue lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 76 kDa why is the actual band size different from the predicted?



    This data was developed using ab274375, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).

    Negative control: PC-12 (PMID: 7842168).

    Exposure time: 3 minutes.

  • Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)
    Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)
    All lanes : Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 76 kDa why is the actual band size different from the predicted?



    This data was developed using ab274375, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).

    Negative control: NIH/3T3 (PMID: 7842168).

    Exposure times: 3 minutes.

  • Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)
    Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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