Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free (ab274382)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23792-163] to Butyrylcholinesterase - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Butyrylcholinesterase antibody [EPR23792-163] - BSA and Azide free
See all Butyrylcholinesterase primary antibodies -
Description
Rabbit monoclonal [EPR23792-163] to Butyrylcholinesterase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse liver, brain, heart and lung tissue lysates; Rat liver and brain tissue lysates.
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General notes
ab274382 is the carrier-free version of ab274375. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23792-163 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?This data was developed using ab274375, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).
This blot was developed using a higher sensitivity ECL substrate.
Exposure times: Lane 1-3: 89 seconds Lane 4: 3 minutes.
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All lanes : Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution
Lane 1 : Rat liver tissue lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?This data was developed using ab274375, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).
Negative control: PC-12 (PMID: 7842168).
Exposure time: 3 minutes.
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All lanes : Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?This data was developed using ab274375, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).
Negative control: NIH/3T3 (PMID: 7842168).
Exposure times: 3 minutes.
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