Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-heavy chain Myosin/MYH3 antibody [F1.652] - BSA and Azide free (ab264039)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-heavy chain Myosin/MYH3 antibody [F1.652] - BSA and Azide free
    See all heavy chain Myosin/MYH3 primary antibodies

  • Description

    Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse embryo tissue lysate; L6 whole cell lysate; Mouse 14.5 limb tissue lysate. IHC-P: Mouse 14.5 tissue; Rat 14.5 tissue.

  • General notes

    ab264039 is the carrier-free version of ab264038.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [AF1.652] - BSA and Azide free (ab264039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [AF1.652] - BSA and Azide free (ab264039)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of mouse E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264038).

  • Western blot - Anti-heavy chain Myosin/MYH3 antibody [AF1.652] - BSA and Azide free (ab264039)
    Western blot - Anti-heavy chain Myosin/MYH3 antibody [AF1.652] - BSA and Azide free (ab264039)
    All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml

    Lane 1 : Mouse embryo tissue lysate
    Lane 2 : Mouse adult skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 224 kDa


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Mouse adult skelatal muscle (PMID:3530499).

    The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264038).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [AF1.652] - BSA and Azide free (ab264039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [AF1.652] - BSA and Azide free (ab264039)

    Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of rat E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264038).

  • Western blot - Anti-heavy chain Myosin/MYH3 antibody [AF1.652] - BSA and Azide free (ab264039)
    Western blot - Anti-heavy chain Myosin/MYH3 antibody [AF1.652] - BSA and Azide free (ab264039)
    All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml

    Lane 1 : L6 (rat skeletal muscle myoblast), whole cell lysate
    Lane 2 : Mouse E14.5 limb tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 224 kDa


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264038).

  • Anti-heavy chain Myosin/MYH3 antibody [F1.652] - BSA and Azide free (ab264039)
    Anti-heavy chain Myosin/MYH3 antibody [F1.652] - BSA and Azide free (ab264039)

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