Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13024(B)] to Choline Acetyltransferase - BSA and Azide free
  • Suitable for: WB, IHC-P, IP, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free
    See all Choline Acetyltransferase primary antibodies

  • Description

    Rabbit monoclonal [EPR13024(B)] to Choline Acetyltransferase - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SH-SY5Y and Human fetal brain lysate. SH-SY5Y cells.

  • General notes

    Ab224267 is the carrier-free version of ab181023. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab224267 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
    Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

    ab181023 (purified) at 1/30 dilution (2ug) immunoprecipitating Choline Acetyltransferase in Human fetal brain lysate. Human fetal brain lysate 10ug
    Lane 2 (+): ab181023 & Human fetal brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181023 in Human fetal brain lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181023).

  • Flow Cytometry - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
    Flow Cytometry - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Choline Acetyltransferase with purified ab181023 at 1/60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181023).

  • Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
    Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

    Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling Choline Acetyltransferase with purified ab181023 at 1/50 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181023).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Choline Acetyltransferase with purified ab181023 at 1/2000 dilution (0.28 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181023).

  • Flow Cytometry - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
    Flow Cytometry - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

    Flow Cytometry analysis of SH-SY5Y cells labeling Choline Acetyltransferase using ab181023 (unpurified) at a 1/110 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181023).

  • Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
    Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

    Lysate from Human fetal brain (Lane 1) and  negative control (Lane 2) were immunoprecipitated with ab181023 (unpurified) at a 1/70 dilution. A anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181023).

  • Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
    Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

    This ICC data was generated using the same anti-Choline Acetyltransferase antibody clone [EPR12024(B)] in a different buffer formulation (cat# ab181023).

    Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling Choline Acetyltransferase using ab181023 (unpurified) at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/100 dilution.

  • Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
    Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.