Anti-Choline Acetyltransferase antibody - C-terminal (ab85609)
Key features and details
- Rabbit polyclonal to Choline Acetyltransferase - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Choline Acetyltransferase antibody - C-terminal
See all Choline Acetyltransferase primary antibodies -
Description
Rabbit polyclonal to Choline Acetyltransferase - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide corresponding to Rat Choline Acetyltransferase aa 600 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab97420) -
Positive control
- This antibody gave a positive signal in both Rat Cortex and Mouse Cholinergic nucleus Tissue Lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).Target
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Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. -
Involvement in disease
Myasthenic syndrome, congenital, 6, presynaptic -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. - Information by UniProt
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Database links
- Entrez Gene: 12647 Mouse
- Entrez Gene: 290567 Rat
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 442817 Mouse
- Unigene: 45116 Rat
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Alternative names
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- ChAT antibody
see all
Images
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Western blot - Anti-Choline Acetyltransferase antibody - C-terminal (ab85609)Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cortex (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-Choline Acetyltransferase antibody - C-terminal (ab85609)Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cholinergic nucleus Mouse Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Datasheets and documents
References (2)
ab85609 has been referenced in 2 publications.
- Xu J et al. Self-Assembling Peptide Scaffold Carrying Neural-Cell Adhesion Molecule-Derived Mimetic-Peptide Transplantation Promotes Proliferation and Stimulates Neurite Extension by Modulating Tau Phosphorylation and Calpain/Glycogen Synthase Kinase 3 beta (GSK-3ß) in Neurons. Ann Transplant 25:e924093 (2020). PubMed: 32686658
- Moghimi E et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One 10:e0126355 (2015). WB ; Mouse . PubMed: 26020962
Images
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Western blot - Anti-Choline Acetyltransferase antibody - C-terminal (ab85609)Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cortex (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
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Western blot - Anti-Choline Acetyltransferase antibody - C-terminal (ab85609)Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cholinergic nucleus Mouse Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
