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Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)

Key features and details

  • Mouse monoclonal [LM609] to Integrin alpha V beta 3
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Integrin alpha V beta 3 antibody [LM609]
    See all Integrin alpha V beta 3 primary antibodies

  • Description

    Mouse monoclonal [LM609] to Integrin alpha V beta 3

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Full length protein corresponding to Integrin alpha V beta 3.

  • Positive control

    • ICC/IF: Malme-3M cells. Flow Cytometry: HUVEC cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)
    Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)

    Overlay histogram showing HUVEC cells stained with ab190147 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab190147) (1 µg/1x106 cells) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min on ice.

    Isotype control antibody (black line) was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)
    Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)

    Overlay histogram showing HUVEC (Human umbilical vein endothelial cell line) cells stained with ab190147 (red line). The cells were fixed with 4% formaldehyde (10 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab190147, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/4000 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab19353, 1 µg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)
    Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)

    ab190147 stained Malme-3M cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab190147 at 5 µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.

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