Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)] (ab78289)
![Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)] (ab78289)](https://www.abcam.com/ps/products/78/ab78289/Images/Integrin-alpha-V-beta-3-Primary-antibodies-ab78289-23.jpg "Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)] (ab78289)")
Key features and details
- Mouse monoclonal [27.1 (VNR-1)] to Integrin alpha V beta 3
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)]
See all Integrin alpha V beta 3 primary antibodies -
Description
Mouse monoclonal [27.1 (VNR-1)] to Integrin alpha V beta 3 -
Host species
Mouse -
Specificity
Reacts with the Integrin alpha V beta 3 -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
27.1 (VNR-1) -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)] (ab78289)
Overlay histogram showing A431 cells stained with ab78289 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78289, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
