Anti-ATP6V1B1 antibody [EPR16400] (ab192612)
![Anti-ATP6V1B1 antibody [EPR16400] (ab192612)](https://www.abcam.com/ps/products/192/ab192612/Images/ab192612-230404-anti-atp6v1b1-antibody-epr16400-immunohistochemistry.gif "Anti-ATP6V1B1 antibody [EPR16400] (ab192612)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16400] to ATP6V1B1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ATP6V1B1 antibody [EPR16400]
See all ATP6V1B1 primary antibodies -
Description
Rabbit monoclonal [EPR16400] to ATP6V1B1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- JAR, Human fetal kidney, Mouse kidney and spleen, Rat kidney lysates; Human, Mouse and Rat kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16400 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)](https://www.abcam.com/ps/products/192/ab192612/Images/ab192612-230403-anti-atp6v1b1-antibody-epr16400-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)](https://www.abcam.com/ps/products/192/ab192612/Images/ab192612-230402-anti-atp6v1b1-antibody-epr16400-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)](https://www.abcam.com/ps/products/192/ab192612/Images/ab192612-230401-anti-atp6v1b1-antibody-epr16400-western-blot.jpg)
Western blot - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)All lanes : Anti-ATP6V1B1 antibody [EPR16400] (ab192612) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa
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![Western blot - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)](https://www.abcam.com/ps/products/192/ab192612/Images/ab192612-230400-anti-atp6v1b1-antibody-epr16400-western-blot.jpg)
Western blot - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)Anti-ATP6V1B1 antibody [EPR16400] (ab192612) at 1/1000 dilution + JAR cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa
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![Western blot - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)](https://www.abcam.com/ps/products/192/ab192612/Images/ab192612-230399-anti-atp6v1b1-antibody-epr16400-western-blot.jpg)
Western blot - Anti-ATP6V1B1 antibody [EPR16400] (ab192612)Anti-ATP6V1B1 antibody [EPR16400] (ab192612) at 1/10000 dilution + Human fetal kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa
-
![Anti-ATP6V1B1 antibody [EPR16400] (ab192612)](https://www.abcam.com/ps/products/192/ab192612/Images/ab192612-7-benefits-of-recombinant-antibodies.png)
Anti-ATP6V1B1 antibody [EPR16400] (ab192612)