Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4B4] to Uroplakin Ia - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free
    See all Uroplakin Ia primary antibodies

  • Description

    Mouse monoclonal [4B4] to Uroplakin Ia - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse bladder, Rat bladder tissues.

  • General notes

    ab255742 is the carrier-free version of ab237776. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255742 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    4B4

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)

    This data was developed using ab237776 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse bladder tissue labeling Uroplakin Ia with ab237776 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in mouse bladder.The section was incubated with ab237776 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)

    This data was developed using ab237776 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat bladder tissue labeling Uroplakin Ia with ab237776 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in rat bladder.The section was incubated with ab237776 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)

    This data was developed using ab237776 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Uroplakin Ia with ab237776 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining in mouse spleen.The section was incubated with ab237776 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)

    This data was developed using ab237776 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Uroplakin Ia with ab237776 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining in rat spleen.The section was incubated with ab237776 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)
    Anti-Uroplakin Ia antibody [4B4] - BSA and Azide free (ab255742)

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