Anti-PODXL antibody [PcMab-47] (ab264542)
![Anti-PODXL antibody [PcMab-47] (ab264542)](https://www.abcam.com/ps/products/264/ab264542/Images/ab264542-2-anti-podxl-antibody-pcmab47-flow-cytometry-parental-hap1.jpg "Anti-PODXL antibody [PcMab-47] (ab264542)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [PcMab-47] to PODXL
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PODXL antibody [PcMab-47]
See all PODXL primary antibodies -
Description
Mouse monoclonal [PcMab-47] to PODXL -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Caco-2 cells. Wild-type HAP1 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
PcMab-47 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-PODXL antibody [PcMab-47] (ab264542)
Flow cytometric analysis of parental HAP1 (Wildtype control human chronic myelogenous leukemia near-haploid cell line, Right) / PODXL KO HAP1 (Left) cells labelling PODXL with ab264542 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Flow Cytometry - Anti-PODXL antibody [PcMab-47] (ab264542)](https://www.abcam.com/ps/products/264/ab264542/Images/ab264542-1-anti-podxl-antibody-pcmab47-flow-cytometry-mdamb468.jpg)
Flow Cytometry - Anti-PODXL antibody [PcMab-47] (ab264542)Flow cytometric analysis of MDA-MB-468 (Human breast adenocarcinoma epithelial cell, Left) / Caco-2 (Human colorectal adenocarcinoma epithelial cell, Right) cells labelling PODXL with ab264542 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Low expression control: MDA-MB-468. (PMID: 28384052).
Gated on viable cells.
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![Anti-PODXL antibody [PcMab-47] (ab264542)](https://www.abcam.com/ps/products/264/ab264542/Images/ab264542-456293-benefitsofrecombinantantibodies.jpg)
Anti-PODXL antibody [PcMab-47] (ab264542)