Anti-XPF antibody (ab76948)
Key features and details
- Rabbit polyclonal to XPF
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XPF antibody
See all XPF primary antibodies -
Description
Rabbit polyclonal to XPF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to a region between residues 855 and 905 of human XPF (AAB07689.1)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab76948 was affinity purified using an epitope specific to XPF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-XPF antibody (ab76948) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 103 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling XPF with ab76948 at 1/200 (1µg/ml). Detection: DAB.
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ab76948 at 1µg/ml staining XPF in HeLa whole cell lysate immunoprecipitated using ab76948 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).