Cytochrome P450 2E1 overexpression 293T lysate (whole cell) (ab94254)
Overview
-
Product name
Cytochrome P450 2E1 overexpression 293T lysate (whole cell) -
General notes
ab94254 is a 293T cell transfected lysate in which Human Cytochrome P450 2E1 has been transiently over-expressed using a pCMV-Cytochrome P450 2E1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
-
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
-
Background
Function: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Similarity: Belongs to the cytochrome P450 family.
Images
-
SDS-PAGE - Cytochrome P450 2E1 overexpression 293T lysate (whole cell) (ab94254)ab94254 at 15μg/lane on an SDS-PAGE gel
-
Western blot - Cytochrome P450 2E1 overexpression 293T lysate (whole cell) (ab94254)All lanes : Anti-Cytochrome P450 2E1 antibody (ab90564) at 1/500 dilution
Lane 1 :Cytochrome P450 2E1 overexpression 293T lysate (whole cell) (ab94254)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
