Anti-Noradrenaline transporter antibody (ab41559)
Key features and details
- Rabbit polyclonal to Noradrenaline transporter
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Noradrenaline transporter antibody
See all Noradrenaline transporter primary antibodies -
Description
Rabbit polyclonal to Noradrenaline transporter -
Host species
Rabbit -
Specificity
Ab41559 does not cross react with dopamine and serotonin transporters. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Rat
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Immunogen
The noradrenaline transporter antibodies were generated against unique N-terminal peptides that are unique to the noradrenaline transporter protein. The antibodies are raised utilizing linear, multiple antigenic peptides or cyclic peptide methodology.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.80
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris glycine, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Noradrenaline transporter antibody (ab41559)Anti-Noradrenaline transporter antibody (ab41559) at 1/250 dilution + rat brain cortical membrane cell lysate at 70 µg
Predicted band size: 70 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 32 kDa. We are unsure as to the identity of these extra bands.
Western Blot with rat brain cortical membranes -
Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline transporter antibody (ab41559)ICC/IF image of ab41559 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41559, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
