Anti-FAK (phospho Y576) antibody (ab226847)
Key features and details
- Rabbit polyclonal to FAK (phospho Y576)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAK (phospho Y576) antibody
See all FAK primary antibodies -
Description
Rabbit polyclonal to FAK (phospho Y576) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis -
Immunogen
Synthetic peptide within Human FAK (internal sequence) (phospho Y576). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q05397 -
Positive control
- WB: HEK-293T, HepG2 and HeLa whole cell extracts. ICC/IF: SK-N-SH cells. IP: HepG2 whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FAK (phospho Y576) antibody (ab226847) at 1/3000 dilution
Lane 1 : Untreated HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : EGF-treated (50 ng/ml for 30 min) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 3 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 4 : EGF-treated (200 ng/ml for 30 min) HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 119 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-FAK (phospho Y576) antibody (ab226847) at 1/500 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with FAK shRNA whole cell extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 119 kDa7.5% SDS-PAGE gel.
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4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells labeling FAK (phospho Y576) (green) using ab226847 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342.
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All lanes : Anti-FAK (phospho Y576) antibody (ab226847) at 1/1000 dilution
Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with CIP (Calf-intestinal alkaline phosphatase), whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 119 kDa5% SDS-PAGE gel.