Anti-Histone H2B (butyryl K5) antibody (ab235429)
Key features and details
- Rabbit polyclonal to Histone H2B (butyryl K5)
- Suitable for: ChIP, WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2B (butyryl K5) antibody
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B (butyryl K5) -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human Histone H2B (butyryl K5).
Database link: P62807 -
Positive control
- ChIP: Chromatin prepared from HeLa cells treated with 30mM sodium butyrate for 4 hours. IP: HEK-293 whole cell lysate. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4 hours). WB: HeLa, HEK-293, A549 and HepG2 whole cell lysates, treated with 30 mM sodium butyrate for 4 hours.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Histone H2B (butyryl K5) antibody (ab235429)All lanes : Anti-Histone H2B (butyryl K5) antibody (ab235429) at 1/100 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 5 : HeLa whole cell lysate, untreated
Lane 6 : HEK-293 whole cell lysate, untreated
Lane 7 : A549 whole cell lysate, untreated
Lane 8 : HepG2 whole cell lysate, untreated
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (butyryl K5) antibody (ab235429)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H2B (butyryl K5) with ab235429 at 1/15 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
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Immunoprecipitation - Anti-Histone H2B (butyryl K5) antibody (ab235429)Histone H2B (butyryl K5) was immunoprecipitated from HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate using 5 µg of ab235429.
Lane 1: Rabbit control IgG in HEK-293 whole cell lysate.
Lane 2: ab235429 IP in HEK-293 whole cell lysate.
Lane 3: HEK-293 whole cell lysate (20 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
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ChIP - Anti-Histone H2B (butyryl K5) antibody (ab235429)HeLa (human epithelial cell line from cervix adenocarcinoma) (106, treated with 30 mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab235429 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
