Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (CD63 / CD9) (ab267480)
Key features and details
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Urine
Overview
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Product name
Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (CD63 / CD9) -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Exosomes 3 = 10% Inter-assay Sample n Mean SD CV% Exosomes 4 = 11% -
Sample type
Urine -
Species reactivity
Reacts with: Human -
Product overview
Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (ab267480) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.
Using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody, the kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
The kit and the measurement of exosomes is useful in the identification of apoptotic cells by flow cytometry.
Visit our FAQs page for tips and troubleshooting.
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Notes
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 25 tests Anti-CD9 PE (Clone VJ1/20) 1 x 125µl Assay Buffer 10X 1 x 40ml Superparamagnetic capture beads (CD63[TEA3/18]) 1 x 2500µl
Images
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Dot-plot gating strategy for acquisition and analysis.
FSC vs SSC (A) and FL3 v s FL4 (B).
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Flow analysis of exosomes.Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A) and human urine (B), were resuspended in PBS and bound to CD63-capture beads during an overnight incubation. The following day the bead-bound exosomes were indirect stained with primary detection antibody (CD9-PE/CD81-PE).
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Dynamic range of the assay analyzed by flow cytometry.Relationship between background noise and specific signal at different exosome concentrations.