Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [SP137] to Rabbit IgG
  • Suitable for: IHC-P
  • Reacts with: Rat, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Rabbit IgG antibody [SP137]
    See all Rabbit IgG primary antibodies

  • Description

    HRP Rabbit monoclonal [SP137] to Rabbit IgG

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    See all applications and species data

  • Immunogen

    Chemical/ Small Molecule corresponding to Rabbit IgG.

  • Positive control

    • IHC-P: Human normal tonsil tissue. Mouse and rat normal spleen tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.4
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.88% PBS
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    SP137

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

    IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND®.

    The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

    IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal mouse spleen*, performed on a Leica BOND®.

    The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

    IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal rat spleen*, performed on a Leica BOND®.

    The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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