Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)

Key features and details

  • Mouse monoclonal [mAbcam 14955] to Histone H3 (phospho S10) - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ELISA
  • Reacts with: Mouse, Human, Drosophila melanogaster
  • Isotype: IgG1

Overview

  • Product name

    Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Mouse monoclonal [mAbcam 14955] to Histone H3 (phospho S10) - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    ab14955 recognises phospho S10 on Histone H3. It recognises a phospho S28 peptide by ELISA, but is not blocked by phospho S28 in WB.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Human, Drosophila melanogaster
    Predicted to work with: Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Indian muntjac, Monkey, Schizosaccharomyces pombe, Zebrafish, Mammals, Tobacco, Chlamydomonas reinhardtii, African green monkey, Aspergillus nidulans, Neurospora crassa

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Control HeLa Histone Prep. Colcemid treated HeLa Histone prep. HeLa cell lysate treated with calyculin A. IHC-P: Human kidney tissue.

  • General notes

    ab238673 is a PBS only version of ab14955.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Hybridomas were prepared and the resulting clones were positively screened by ELISA against the immunising tri methyl K9 and phospho S10 dimodified peptide. Clones were also positively screened against both tri methyl K9 and phospho S10 peptides. Clones were negatively screened against the unmodified version of the peptides. This clone binds to the tri methyl K9 and phospho S10 dimodified peptide and to the phospho S10 peptide, but not to the tri methyl K9 peptide or to equivalent unmodified Histone H3 peptide.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    mAbcam 14955

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)
    Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)
    All lanes : Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) at 1 µg/ml

    Lane 1 : Control HeLa Histone Prep
    Lane 2 : Colcemid treated HeLa Histone prep
    Lane 3 : Control HeLa Histone Prep with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644) at 1 µg/ml
    Lane 4 : Colcemid treated HeLa Histone prep with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644) at 1 µg/ml
    Lane 5 : Control HeLa Histone Prep with Human Histone H3 (unmodified) peptide (ab7228) at 1 µg/ml
    Lane 6 : Colcemid treated HeLa Histone prep with Human Histone H3 (unmodified) peptide (ab7228) at 1 µg/ml
    Lane 7 : Control HeLa Histone Prep with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg/ml
    Lane 8 : Colcemid treated HeLa Histone prep with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg/ml
    Lane 9 : Control HeLa Histone Prep with Human Histone H3 (phospho S28) peptide (ab14793) at 1 µg/ml
    Lane 10 : Colcemid treated HeLa Histone prep with Human Histone H3 (phospho S28) peptide (ab14793) at 1 µg/ml

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Mouse IgG H&L (HRP) at 1/3000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different formulation containing PBS and Azide (ab14955).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)

    IHC image of ab14955 staining Histone H3 (phospho S10) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14955, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different formulation containing PBS and Azide (ab14955).

  • ELISA - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)
    ELISA - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)

    By ELISA, ab14955 detects: the singly modified phospho S10 peptide and the dual modified phospho S10 and tri methyl K9 peptide (the orange and grey lines respectively), less strongly detects the phospho S28 peptide (purple line), does not detect the equivalent non-modified Histone H3 peptide for S10 or the singly modified tri methyl K9 peptide (the 2 lines at the bottom of the figure).

    The antibody recognises phospho S28 by ELISA (although a phospho S28 peptide does not block the antibody in Western blotting).

    This data was developed using the same antibody clone in a different formulation containing PBS and Azide (ab14955).

  • Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)
    Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)
    All lanes : Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) at 0.5 µg/ml

    Lane 1 : Control HeLa Histone prep
    Lane 2 : Colecemid treated HeLa Histone prep
    Lane 3 : Control HeLa Histone prep with Human Histone H3 (unmodified) peptide (ab7228) at 1 µg
    Lane 4 : Colecemid treated HeLa Histone prep with Human Histone H3 (unmodified) peptide (ab7228) at 1 µg/ml
    Lane 5 : Control HeLa Histone prep with Human Histone H3 (phospho S28) peptide (ab14793) at 1 µg/ml
    Lane 6 : Colecemid treated HeLa Histone prep with Human Histone H3 (phospho S28) peptide (ab14793) at 1 µg
    Lane 7 : Control HeLa Histone prep with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg/ml
    Lane 8 : Colecemid treated HeLa Histone prep with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg/ml

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

    Predicted band size: 15 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different formulation containing PBS and Azide (ab14955).
  • Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)
    Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free (ab238673)
    All lanes : Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) at 1/2000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HeLa cell lysate treated with calyculin A
    Lane 3 : HeLa cell lysate treated with calyculin A and alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/20000 dilution

    Predicted band size: 15 kDa



    This data was developed using the same antibody clone in a different formulation containing PBS and Azide (ab14955).

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