Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)
![Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)](https://www.abcam.com/ps/products/255/ab255687/Images/ab255687-4-anti-glypican-1-gpc1-antibody-epr22580-72-immunohistochemistry-pancreatic-carcinoma-and-the-paired-adjacent-pancreas-human.jpg "Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22580-72] to Glypican 1/ GPC1 - BSA and Azide free
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free
See all Glypican 1/ GPC1 primary antibodies -
Description
Rabbit monoclonal [EPR22580-72] to Glypican 1/ GPC1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: DU 145 (cultured under serum-free conditions), PC-3, MCF7 and MDA-MB-231 whole cell lysates. IHC-P: Human pancreatic carcinoma, lung carcinoma and esophagus carcinoma tissue. IP: DU145 whole cell lysate.
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General notes
Ab255687 is the carrier-free version of ab255600. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255687 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22580-72 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)
Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue (A) and the paired adjacent pancreas tissue (B) labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic and membranous staining on human pancreatic carcinoma (panel A) and no staining on the paired adjacent pancreas (panel B, PMID: 28440066) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255600 for 30 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255600).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)](https://www.abcam.com/ps/products/255/ab255687/Images/ab255687-3-anti-glypican-1-gpc1-antibody-epr22580-72-immunohistochemistry-lung-carcinoma-and-the-paired-adjacent-lung-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue (A) and the paired adjacent lung tissue (B) labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human lung carcinoma (panel A) and the paired adjacent lung (panel B) was barely stained (PMID: 29785020) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255600 for 30 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255600).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)](https://www.abcam.com/ps/products/255/ab255687/Images/ab255687-2-anti-glypican-1-gpc1-antibody-epr22580-72-immunohistochemistry--esophagus-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)Immunohistochemical analysis of paraffin-embedded esophagus lung carcinoma tissue labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on esophagus carcinoma (PMID: 28445969) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255600 for 30 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255600).
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![Immunoprecipitation - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)](https://www.abcam.com/ps/products/255/ab255687/Images/ab255687-1-anti-gpc1-antibody-epr22580-72-immunoprecipitation-du145-human.jpg)
Immunoprecipitation - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)Glypican 1/ GPC1 was immunoprecipitated from 0.35 mg DU 145 (human prostate carcinoma cell line) whole cell lysate 10μg with ab255600 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255600 1/1000 dilution (0.65 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: DU 145 (human prostate carcinoma cell line) whole cell lysate 10μg
Lane 2: ab255600 IP in DU 145 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255600 in DU 145 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 75 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255600).
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![Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)](https://www.abcam.com/ps/products/255/ab255687/Images/ab255687-5-benefits-of-recombinant-antibodies.png)
Anti-Glypican 1/ GPC1 antibody [EPR22580-72] - BSA and Azide free (ab255687)
